Generate positive and negative populations from single stain histograms In this example, the FITC single stain has been gated for lymphocytes, then displayed as a FITC histogramĬlick and use the histogram bisector tool to quickly separate positive from negativeĭrag and drop gated populations to define the matrix Drag your gated populations to the “AutoAssign Box” amd FlowJo will sort out which population goes in the right Definitions box. FlowJo organizes all of your analyses into a workspace.
FlowJo expects to see defined positive and negative populations for each single stain Compensation Matrix File When the 'Compute' button in the compensation matrix dialog window is clicked, FlowJo generates a compensation matrix. A manual adjustment compensation shown below: 2 manual adjustment compensation: i.e.The put your experimental tubes into a separate groupĪccessing FlowJo’s compensation controls.First place your “comp” tubes into a separate group.Using FlowJo to calculate compensation 060502 Emory Vaccine Center FCC / Suzanne Mertens / can be used to calculate compensation for multi-color data acquired on a Calibur Also what I realized that the matrix built-in Kaluza is spectral compensation so the numbers don't work in FLowjo, there is a separate.